Rational Design of a siRNA Delivery System: ALOX5 and Cancer Stem Cells as Therapeutic Targets
Rafael D, Andrade F, Montero S, Gener P, Seras-Franzoso J, Martínez F, González P, Florindo H, Arango D, Sayós J, Abasolo I, Videira M, and Schwartz Jr. S. , Prec. Nanomed. 2018 July;1(2):86-105 DOI:10.29016/180629.1 https://precisionnanomedicine.com/article/12 Abstract The search for an ideal gene delivery system is a long and laborious process in which several factors from
Bacterial cellulose hydrogel loaded with lipid nanoparticles for localized cancer treatment
Cacicedo ML, Islan GA, León IE, Álvarez VA, Chourpa I, Allard-Vannier E, García-Aranda N, Díaz-Riascos ZV, Fernández Y, Schwartz S Jr, Abasolo I, Castro GR Colloids and surfaces. B, Biointerfaces Volumen: 170 Páginas: 596-608 Fecha de publicación: 2018-Jun-26 (Epub 2018 Jun 26) https://www.ncbi.nlm.nih.gov/pubmed/29975908?dopt=Abstract Abstract The use of hybrid materials, where a matrix sustains nanoparticles controlling the
Detection of plasma MMP-9 within minutes. Unveiling some of the clues to develop fast and simple electrochemical magneto-immunosensors
Gisela Ruiz-Vega , Alicia García-Robaina, Manel Ben Ismail, Helena Pasamar, Teresa García-Berrocoso, Joan Montaner, Mohammed Zourob, Ali Othmane, F. Javier del Campo, Eva Baldrich Biosensors and Bioelectronics Volume 115, 15 September 2018, Pages 45-52 https://doi.org/10.1016/j.bios.2018.05.020 Abstract Magnetic beads (MB) have been extensively used to produce sensitive and efficient electrochemical magneto-immunosensors. However, MB effective handling requires
Using polyHRP to produce simplified immunoassays and electrochemical immunosensors. Application to MMP-9 detection in plasma and uterine aspirates
Erica de la Serna, Elena Martínez-García, Teresa García-Berrocoso, Anna Penalba, Antonio Gil-Moreno, Eva Colas, Joan Montaner, Eva Baldrich Sensors and Actuators B: Chemical Volume 269, 15 September 2018, Pages 377-384 https://doi.org/10.1016/j.snb.2018.05.003 Abstract Immunoassays and immunosensors are extensively described diagnostic tools. However, they often entail time-consuming multi-step procedures that are difficult to perform outside centralized diagnostic
Book – Nanoparticles in Life Sciences and Biomedicine
Book – Nanoparticles in Life Sciences and Biomedicine The creation of new and more efficient therapies for improving human health greatly depends on drug delivery systems. Nanotechnology has emerged as a powerful strategy for the development of nanoparticles, such as nanoemulsions, liposomes, nanocrystals, and nanocomplexes, applied in the diagnosis, treatment, or theranostics of several
NaNO3/NaCl Oxidant and Polyethylene Glycol (PEG) Capped Gold Nanoparticles (AuNPs) as a Novel Green Route for AuNPs Detection in Electrochemical Biosensors.
Marzo AM, Hoyos-de-la-Torre R, Baldrich E. Anal Chem. 2018 Mar 20;90(6):4010-4018. doi: 10.1021/acs.analchem.7b05150. Epub 2018 Mar 9.López- https://www.ncbi.nlm.nih.gov/pubmed/29498271?dopt=Abstract Abstract Gold nanoparticles (AuNPs) have been exploited as signal-producing tags in electrochemical biosensors. However, the electrochemical detection of AuNPs is currently performed using corrosive acid solutions, which may raise health and environmental concerns. Here, oxidant salts, and
Efficient EFGR mediated siRNA delivery to breast cancer cells by Cetuximab functionalized Pluronic® F127/Gelatin
Diana Rafael, Francesc Martínez, Fernanda Andrade, Joaquim Seras-Franzoso, Natalia Garcia-Aranda, Pertra Gener, Joan sayós, Diego Arango, Ibane Abasolo, Simó Schwartz Jr. Chemical Engineering Journal https://www.sciencedirect.com/science/article/pii/S1385894717322374 Abstract New polymeric biomaterials and nanomedicines targeting cancer cells are highly required because of their potential clinical applications. Gene therapy enjoys high popularity as advanced therapy due to its high
Improved pharmacokinetic profile of lipophilic anti-cancer drugs using ανβ3-targeted polyurethane-polyurea nanoparticles.
Rocas P, Fernández Y, García-Aranda N, Foradada L, Calvo P, Avilés P, Guillén MJ, Schwartz S Jr., Rocas J, Albericio F, Abasolo I. Nanomedicine. 2018 Feb;14(2):257-267. doi: 10.1016/j.nano.2017.10.009. Epub 2017 Nov 7. https://www.ncbi.nlm.nih.gov/pubmed/?term=Improved+Pharmacokinetic+Profile+of+Lipophilic+Anti-Cancer+Drugs+Using+%CE%B1%CE%BD%CE%B23-targeted+Polyurethane-Polyurea+Nanoparticles Abstract Glutathione degradable polyurethane-polyurea nanoparticles (PUUa NP) with a disulfide-rich multiwalled structure and a cyclic RGD peptide as a targeting moiety were
TNFα-senescence initiates a STAT-dependent positive feedback loop, leading to a sustained interferon signature, DNA damage, and cytokine secretion.
Kandhaya-Pillai R, Miro-Mur F, Alijotas-Reig J, Tchkonia T, Kirkland JL, Schwartz S Aging (Albany NY). 2017 Nov 22;9(11):2411-2435. doi: 10.18632/aging.101328. https://www.ncbi.nlm.nih.gov/pubmed/29176033 Abstract Cellular senescence is a cell fate program that entails essentially irreversible proliferative arrest in response to damage signals. Tumor necrosis factor-alpha (TNFα), an important pro-inflammatory cytokine secreted by some types of senescent cells,
Effect of Specific Mutations in Cd300 Complexes Formation; Potential Implication of Cd300f in Multiple Sclerosis.
Martínez-Barriocanal Á, Arcas-García A, Magallon-Lorenz M, Ejarque-Ortíz A, Negro-Demontel ML, Comas-Casellas E, Schwartz S Jr, Malhotra S, Montalban X, Peluffo H, Martín M, Comabella M, Sayós J. Scientific Reports, 2017 Oct 19;7(1):13544. https://www.ncbi.nlm.nih.gov/pubmed/?term=Effect+of+Specific+Mutations+in+Cd300+Complexes+Formation%3B+Potential+Implication+of+Cd300f+in+Multiple+Sclerosis. Abstract Herein, we have used bioinformatics tools to predict five clusters defining ligand-binding sites on the extracellular domain of human CD300b receptor, presumably
Diverse Applications of Nanomedicine.
Pelaz B, Alexiou C, Alvarez-Puebla RA, Alves F, Andrews AM, Ashraf S, Balogh LP, Ballerini L, Bestetti A, Brendel C, Bosi S, Carril M, Chan WC, Chen C, Chen X, Chen X, Cheng Z, Cui D, Du J, Dullin C, Escudero A, Feliu N, Gao M, George M, Gogotsi Y, Grunweller A, Gu Z, Halas
Effect of agitation in magneto-assay performance
G. Ruiz-Vega, E. Baldrich. Sensors and Actuators B: Chemical , 247, 718–726. http://www.sciencedirect.com/science/article/pii/S092540051730494X Magnetic particles (MP) are extensively used to improve immunocapture and immunodetection. Com-pared to two-dimensional surfaces, MP provide wider active areas, efficient mixing with the sample,and target separation from other sample components. Nevertheless, MP handling is more tedious thanclassical techniques, such as
Loss of the EPH receptor B6 contributes to colorectal cancer metastasis.
Mateo-Lozano S, Bazzocco S, Rodrigues P, Mazzolini R, Andretta E, Dopeso H, Fernandez Y, Del Llano E, Bilic J, Suarez-Lopez L, Macaya I, Carton-Garcia F, Nieto R, Jimenez-Flores LM, de Marcondes PG, Nunez Y, Afonso E, Cacci K, Hernandez-Losa J, Landolfi S, Abasolo I, Ramon Y Cajal S, Mariadason JM, Schwartz S Jr, Matsui T,
Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer
Andretta E, Carton-Garcia F, Martinez-Barriocanal, de Marcondes PG, Jimenez-Flores LM, Macaya I, Bazzocco S, Bilic J, Rodrigues P, Nieto R, Landolfi S, Ramon Y Cajal S, Schwartz S, Brown A, Dopeso H, Arango D Sci Rep. 2017 Feb 7;7:41576. https://www.ncbi.nlm.nih.gov/pubmed/28169277?dopt=Abstract Abstract EPH signaling deregulation has been shown to be important for colorectal carcinogenesis and genome-wide sequencing
Unmet needs in developing nanoparticles for precision medicine
Schwartz S Jr Nanomedicine (Lond). 2017 Feb;12(4):271-274. doi: 10.2217/nnm-2016-0390. Epub 2017 Jan 17. https://www.ncbi.nlm.nih.gov/pubmed/?term=28093937
Nonclinical Evaluation of the New Topical Hemostatic Agent TT-173 for Skin Grafting Procedures.
Codina SR, Herance JR, Centeno A, Valero J, Arias B, Miquel I, Sánchez P, Rincón E, López R, Murat J. J Burn Care Res. 2017 Jan 31. doi: 10.1097/BCR.0000000000000497 https://www.ncbi.nlm.nih.gov/pubmed/?term=28157787 Abstract Blood loss during grafting surgery represents a major concern of this procedure and the development of hemostatic agents for this indication is highly desirable.
Size-Dependent Protein-Nanoparticle Interactions in Citrate-Stabilized Gold Nanoparticles: The Emergence of the Protein Corona.
Piella J, Bastús NG, Puntes V. Bioconjug Chem. 2017 Jan 18;28(1):88-97. doi: 10.1021/acs.bioconjchem.6b00575. Epub 2016 Dec 20. https://www.ncbi.nlm.nih.gov/pubmed/?term=27997136 Abstract Surface modifications of highly monodisperse citrate-stabilized gold nanoparticles (AuNPs) with sizes ranging from 3.5 to 150 nm after their exposure to cell culture media supplemented with fetal bovine serum were studied and characterized by the combined
Ultrasound functional evaluation of fetuses with myelomeningocele: study of the interpretation of results.
Maroto A, Illescas T, Melendez M, Arevalo S, Rodo C, Peiro JL, Belfort M, Cuxart A, Carreras E J Matern Fetal Neonatal Med. 2017 Jan 16:1-5. doi: 10.1080/14767058.2016.1247262. https://www.ncbi.nlm.nih.gov/pubmed/27997256?dopt=Abstract Abstract OBJECTIVE: To assess the reliability of the interpretation of a new technique for the ultrasound evaluation of the level of neurological lesion in fetuses
Design of chalcogen-containing norepinephrines: efficient GPx mimics and strong cytotoxic agents against HeLa cells
Marset A, Begines P, López Ó, Maya I, García-Aranda N, Schwartz S Jr, Abasolo I, Fernández-Bolaños JG. Future Med Chem. 2016 Dec;8(18):2185-2195. Epub 2016 Nov 15. https://www.ncbi.nlm.nih.gov/pubmed/27845568 Abstract AIM: Numerous chronic diseases exhibit multifactorial etiologies, so focusing on a single therapeutic target is usually an inadequate treatment; instead, multi-target drugs are preferred. Herein, a panel
Toxicological properties of two fluorescent carbon quantum dots with onion ring morphology and their usefulness as bioimaging agents
Ana Blas-Garcia, Herme G. Baldoví, Miriam Polo, Victor M. Victor, Hermenegildo Garcia and Jose Raul Herance RSC Advances, Issue 36, 2016, http://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C5RA27662D#!divAbstract Abstract In the present work, two carbon quantum dots with onion ring morphology, C-NOR and C-NOR(Eu) with an average size of 40 nm differing in the absence or presence of Eu3+ as Lewis
Bacterial mimetics of endocrine secretory granules as immobilized in vivo depots for functional protein drugs
Céspedes MV, Fernández Y, Unzueta U, Mendoza R, Seras-Franzoso J, Sánchez-Chardi A, Álamo P, Toledo-Rubio V, Ferrer-Miralles N, Vazquez E, Schwartz S Jr, Abasolo I, Corchero JL, Mangues R, Villaverde A Sci Rep. 2016 Oct 24;6:35765. http://www.nature.com/articles/srep35765 Abstract In the human endocrine system many protein hormones including urotensin, glucagon, obestatin, bombesin and secretin, among others,
Highly versatile polyelectrolyte complexes for improving the enzyme replacement therapy of lysosomal storage disorders.
Giannotti, Marina; Abasolo, Ibane; Oliva, Mireia; Andrade, Fernanda; García-Aranda, Natalia; Melgarejo, Marta; Pulido, Daniel; Corchero, José; Fernández, Yolanda; Villaverde, Antonio; Royo, Miriam; Garcia-Parajo, Maria; Sanz, Fausto; Schwartz S Jr. ACS Applied Materials and Interfaces, 2016 Oct 5;8(39):25741-25752. https://www.ncbi.nlm.nih.gov/pubmed/27610822 Abstract Lysosomal storage disorders are currently treated by enzyme replacement therapy (ERT) through the direct administration of
Detection of Uric Acid at Reversibly Nanostructured Thin-Film Microelectrodes.
Z Herrasti, F Martínez, E Baldrich (2016) Sens Act B, 234:667–673 Sensors and Actuators B: Chemical Volume 234, 29 October 2016, Pages 667–673 http://dx.doi.org/10.1016/j.snb.2016.05.018 Abstract Nanomaterials such as carbon nanotubes (CNT) are extensively used to produce electrodes of enhanced performance. However, since their incorporation tends to be irreversible, nanostructured electrodes are used only once or
Assessing the Immunosafety of Engineered Nanoparticles with a Novel in Vitro Model Based on Human Primary Monocytes.
Li Y, Italiani P, Casals E, Valkenborg D, Mertens I, Baggerman G, Nelissen I, Puntes VF, Boraschi D. ACS Appl Mater Interfaces. 2016 , 8 (42), pp 28437–28447 https://www.ncbi.nlm.nih.gov/pubmed/?term=27670583 Abstract The possibility that nanomaterials could perturb the normal course of an inflammatory response is a key issue when assessing nanoimmunosafety. The alteration of the normal progress
One-pot polyol synthesis of highly monodisperse short green silver nanorods.
Patarroyo J, Genç A, Arbiol J, Bastús NG, Puntes V. Chem Commun (Camb). 2016 Sep 21;52(73):10960-3. https://www.ncbi.nlm.nih.gov/pubmed/?term=27498830 Abstract Green silver nanorods (Ag NRs) of a low aspect ratio (2.8) have been produced in high yields via an optimized, simple, and robust one-pot polyol method in the presence of tannic acid, which favors the nucleation of
Characterization of the Biodistribution and Systemic Absorption of TT-173, a New Hemostatic Agent of Recombinant Human Tissue Factor, Using Radiolabeling with 18F.
Rojas S, Herance JR, Gispert JD, Arias B, Miquel I, López R, Sánchez P, Rincón E, Murat J Eur J Drug Metab Pharmacokinet. 2016 Aug 22. [Epub ahead of print] https://www.ncbi.nlm.nih.gov/pubmed/?term=27550654 Abstract BACKGROUND AND OBJECTIVES: TT-173 is the first topical hemostatic agent based on tissue factor. To prevent thromboembolic events and intravascular coagulation it
Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria
Pesarrodona M1, Fernández Y, Foradada L, Sánchez-Chardi A, Conchillo-Solé O, Unzueta U, Xu Z, Roldán M, Villegas S, Ferrer-Miralles N, Schwartz S Jr, Rinas U, Daura X, Abasolo I, Vázquez E, Villaverde A. Biofabrication. 2016 Apr 14;8(2):025001 https://www.ncbi.nlm.nih.gov/pubmed/?term=Conformational+and+functional+variants+of+CD44-targeted+protein+nanoparticles+bio-produced+in+bacteria Abstract Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational
Alpha-Galactosidase A Loaded Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration.
I Cabrera, I Abasolo, JL.Corchero, E Elizondo, P Rivera Gil, E Moreno, J Faraudo, S Sala, D Bueno, E González-Mira, M Rivas, M Melgarejo, D Pulido, F Albericio, M Royo, A Villaverde, M F. García-Parajo, Schwartz S Jr., N Ventosa, J Veciana Advanced Healthcare Materials. 2016 Apr 6;5(7):829-40 https://www.ncbi.nlm.nih.gov/pubmed/?term=Alpha-Galactosidase+A+Loaded+Nanoliposomes+with+Enhanced+Enzymatic+Activity+and+Intracellular+Penetration. Abstract Lysosomal storage disorders (LSD) are
Increased secretory sphingomyelinase activity in the first trimester of pregnancy in women later developing preeclampsia: a nested case-control study.
Rodríguez-Sureda V, Crovetto F, Triunfo S, Sánchez O, Crispi F, Llurba E, Gratacós E, Figueras F, Domínguez C. Biol Chem. 2016 Mar;397(3):269-79. https://www.ncbi.nlm.nih.gov/pubmed/?term=26756094 Abstract The pathogenic basis of abnormal placentation and dysfunction in preeclampsia (PE) is highly complex and incompletely understood. Secretory sphyngomyelinase activity (S-ASM) was analyzed in plasma samples from 158 pregnant women developing
A New Surgical Model of Skeletal Muscle Injuries in Rats Reproduces Human Sports Lesions.
Contreras-Muñoz P, Fernández-Martín A, Torrella R, Serres X, De la Varga M, Viscor G, Järvinen TA, Martínez-Ibáñez V, Peiró JL, Rodas G, Marotta M. Int J Sports Med. 2016 Mar;37(3):183-90. https://www.ncbi.nlm.nih.gov/pubmed/?term=A+New+Surgical+Model+of+Skeletal+Muscle+Injuries+in+Rats+Reproduces+Human+Sports+Lesions. Abstract Skeletal muscle injuries are the most common sports-related injuries in sports medicine. In this work, we have generated a new surgically-induced skeletal muscle
Prenatal ultrasound evaluation of segmental level of neurological lesion in fetuses with myelomeningocele: development of a new technique
E. Carreras, A. Maroto, T. Illescas, M. Meléndez, S. Arévalo, J. L. Peiró,C. G. García-Fontecha, M. Belfort, A. Cuxart ULTRASOUND OBST GYN Volume 47, Issue 2, February 2016 Pages 162–167 http://onlinelibrary.wiley.com/doi/10.1002/uog.15732/abstract;jsessionid=CFF3B04EE42373E15C7C000FDABCBD92.f04t02 ABSTRACT Objectives To report our preliminary experience in the use of prenatal ultrasound examination to assess lower-limb movements in fetuses with myelomeningocele. We aimed to
Self-assembly PEGylation assists SLN-Paclitaxel delivery inducing cancer cell apoptosis upon internalization.
Arranja A, Gouveia LF, Gener P, Rafael DF, Pereira C, Schwartz S Jr, Videira MA. Int J Pharmatech 2016 Feb 4. pii: S0378-5173(16)30075-8. http://www.sciencedirect.com/science/article/pii/S0378517316300758 Abstract In past years, a considerable progress has been made in the conversion of conventional chemotherapy into potent and safe nanomedicines. The ultimate goal is to improve the therapeutic window of
Cancer stem cells and personalized cancer nanomedicine
Gener P, Rafael DF, Fernandez Y, Ortega JS, Arango D, Abasolo I, Videira M, Schwartz S Jr. Nanomedicine (Lond) 2016 Feb; 11(3):307-20 https://www.ncbi.nlm.nih.gov/pubmed/26785724 Abstract Despite the progress in cancer treatment over the past years advanced cancer is still an incurable disease. Special attention is pointed toward cancer stem cell (CSC)-targeted therapies, because this minor cell
Miniaturized metal oxide pH sensors for bacteria detection
N Uria, N Abramova, A Bratov, FX Muñoz-Pascual, E Baldrich Talanta, Volume 147, 15 January 2016, Pages 364–369 http://www.sciencedirect.com/science/article/pii/S0039914015303775 Abstract It is well known that the metabolic activity of some microorganisms results in changes of pH of the culture medium, a phenomenon that can be used for detection and quantification of bacteria. However, conventional glass
Developing enhanced magneto-immunosensors based on low-cost screen-printed electrode devices
Z Herrasti, E de la Serna, G Ruiz-Vega, E Baldrich Developing enhanced magneto-immunosensors based on low-cost screen-printed electrode devices. Reviews in Analytical Chemistry, (2016) 35: 53–85. Artículo de revisión invitado. https://www.researchgate.net/publication/304923361_Developing_enhanced_magnetoimmunosensors_based_on_low-cost_screen-printed_electrode_devices Abstract Electrochemical magnetoimmunosensors combine a number of issues that guarantee extremely high performance and also compatibility with the study of complex sample matrices. First,
Fluorescent CSC models evidence that targeted nanomedicines improve treatment sensitivity of breast and colon cancer stem cells.
Gener P, Gouveia LP, Sabat GR, de Sousa Rafael DF, Fort NB, Arranja A, Fernández Y, Prieto RM, Ortega JS, Arango D, Abasolo I, Videira M, Schwartz S Jr. Nanomedicine. 2015 Nov;11(8):1883-92. https://www.ncbi.nlm.nih.gov/pubmed/?term=Fluorescent+CSC+models+evidence+that+targeted+nanomedicines+improve+treatment+sensitivity+of+breast+and+colon+cancer+stem+cells. Abstract To be able to study the efficacy of targeted nanomedicines in marginal population of highly aggressive cancer stem cells (CSC), we
Biological assessment of self-assembled polymeric micelles for pulmonary administration of insulin.
Andrade F, Neves Jd, Gener P, Schwartz S Jr, Ferreira D, Oliva M, Sarmento B. Nanomedicine-NBM. 2015 Oct;11(7):1621-31 https://www.ncbi.nlm.nih.gov/pubmed/26049134 Abstract Pulmonary delivery of drugs for both local and systemic action has gained new attention over the last decades. In this work, different amphiphilic polymers (Soluplus®, Pluronic® F68, Pluronic® F108 and Pluronic® F127) were used to
Improving the Diagnosis of Infective Endocarditis in Prosthetic Valves and Intracardiac Devices With 18F-Fluordeoxyglucose Positron Emission Tomography/Computed Tomography Angiography: Initial Results at an Infective Endocarditis Referral Center.
Pizzi MN, Roque A, Fernández-Hidalgo N, Cuéllar-Calabria H, Ferreira-González I, Gonzàlez-Alujas MT, Oristrell G, Gracia-Sánchez L, González JJ, Rodríguez-Palomares J, Galiñanes M, Maisterra-Santos O, Garcia-Dorado D, Castell-Conesa J, Almirante B, Aguadé-Bruix S, Tornos P. Circulation. 2015 Sep 22;132(12):1113-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=Improving+the+Diagnosis+of+Infective+Endocarditis+in+Prosthetic+Valves+and+Intracardiac+Devices+With+18F-Fluordeoxyglucose+Positron+Emission+Tomography%2FComputed+Tomography+Angiography%3A+Initial+Results+at+an+Infective+Endocarditis+Referral+Center. Abstract BACKGROUND: The diagnosis of infective endocarditis (IE) in prosthetic valves and intracardiac devices is challenging
Ceria nanoparticles with rhodamine B as a powerful theranostic agent against intracellular oxidative stress.
Nadezda Apostolova; Susana Rovira-Llopis; Herme G Baldoví; Sergio Navalon; Abdullah M. Asiri; Hermenegildo Garcia; José Raúl Herance. RSC Advances., 2015,5, 79423-79432 http://pubs.rsc.org/en/Content/ArticleLanding/2015/RA/C5RA12794G#!divAbstract Abstract Ceria nanoparticles with rhodamine B groups covalently attached on their surface (RhB-CeNPs) were successfully prepared to simultaneously exhibit antioxidant activity and the ability to detect oxidant species. In order to use them
A novel bio-functional material based on mammalian cell aggresomes
Rodríguez-Carmona E, Mendoza R, Ruiz-Cánovas E, Ferrer-Miralles N, Abasolo I, Schwartz S Jr, Villaverde A, Corchero JL. Appl Microbiol Biotechnol. 2015 Sep;99(17):7079-88 https://www.ncbi.nlm.nih.gov/pubmed/?term=A+novel+bio-functional+material+based+on+mammalian+cell+aggresomes Abstract Aggresomes are protein aggregates found in mammalian cells when the intracellular protein degradation machinery is over-titered. Despite that they abound in cells producing recombinant proteins of biomedical and biotechnological interest, the
Multifunctionalized polyurethane–polyurea nanoparticles: hydrophobically driven selfstratification at the o/w interface modulates encapsulation stability
Pau Rocas, Yolanda Fernandez, Schwartz S Jr, Ibane Abasolo, Josep Rocas and Fernando Albericio . Journal of Materials Chemistry B. 2015,3, 7604-7613 http://pubs.rsc.org/en/content/articlelanding/tb/2015/c5tb01345c#!divAbstract Abstract Polyurethane–polyurea (PUUa) reactive prepolymers with adjusted hydrophobic and hydrophilic dangling chains to achieve multiwalled sub-30 nm nanoparticles are presented. The combination of an amphiphilic and a hydrophobic prepolymer at the
Reversible nanostructuration of microfluidic electrode devices by CNT magnetic co-entrapment
Z Herrasti, F Martínez, E Baldrich Lab on a Chip, 2015 Aug 21;15(16):3269-73 https://www.ncbi.nlm.nih.gov/pubmed/?term=Reversible+nanostructuration+of+microfluidic+electrode+devices+by+CNT+magnetic+co-entrapment Abstract Carbon nanotubes (CNTs) have been extensively used to produce electrodes of enhanced performance but have only been very recently exploited in microfluidic devices. In these cases, CNT electrodes had to be produced prior to device assembly, which might damage the CNT
Diosgenin-based thio(seleno)ureas and triazolyl glycoconjugates as hybrid drugs. Antioxidant and antiproliferative profile
Simó Schwartz Navarro Romero-Hernández LL, Merino-Montiel P, Montiel-Smith S, Meza-Reyes S, Vega-Báez JL, Abasolo I, Schwartz S Jr, López Ó, Fernández-Bolaños JG. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Pages : 99:67-81 Date : 2015-08 https://www.ncbi.nlm.nih.gov/pubmed/26046314 Abstract The stereoselective preparation of diosgenin-derived thio(seleno)ureas and glycomimetics bearing a 1,2,3-triazolyl tether on C-3 has been accomplished. The key
Highly Expressed Genes in Rapidly Proliferating Tumor Cells as New Targets for Colorectal Cancer Treatment.
Bazzocco S, Dopeso H, Carton-Garcia F, Macaya I, Andretta E, Chionh F, Rodrigues P, Garrido M, Alazzouzi H, Nieto R, Sanchez A, Schwartz S Jr, Bilic J, Mariadason JM, Arango D. Clin Cancer Res. 2015 Aug 15;21(16):3695-704. https://www.ncbi.nlm.nih.gov/pubmed/25944804 Abstract PURPOSE: The clinical management of colorectal cancer patients has significantly improved because of the identification of
Perylenetetracarboxylic anhydride as a precursor of fluorescent carbon nanoonion rings.
Baldoví HG, Herance JR, Manuel Víctor V, Alvaro M, Garcia H. Nanoscale. 2015 Aug 7;7(29):12484-91. doi: 10.1039/c5nr02903a. Epub 2015 Jul 2. https://www.ncbi.nlm.nih.gov/pubmed/?term=26135910 Abstract Thermal annealing at 400 °C of perylenetetracarboxylic anhydride in low molecular mass PEG gives rise to the formation of well defined nanoobjects of 2.5 nm height and size distribution from 10 to
Myo5b knockout mice as a model of microvillus inclusion disease
Cartón-García F, Overeem AW, Nieto R, Bazzocco S, Dopeso H, Macaya I, Bilic J, Landolfi S, Hernandez-Losa J, Schwartz S Jr, Ramon y Cajal S, van Ijzendoorn SC, Arango D. 2015 Scientific Reports, Jul 23;5:12312 http://www.nature.com/articles/srep12312 Abstract: Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized
Dual chronoamperometric detection of enzymatic biomarkers using magnetic beads and a low-cost flow cell
J Moral-Vico, J Barallat, Ll Abad, R Olivé-Monllau, FX Muñoz-Pascual, A Galán Ortega, FJ del Campo, E Baldrich* Biosens Bioelectron, 2015 Jul 15;69:328-36 https://www.ncbi.nlm.nih.gov/pubmed/?term=Dual+chronoamperometric+detection+of+enzymatic+biomarkers+using+magnetic+beads+and+a+low-cost+flow+cell Abstract In this work we report on the production of a low cost microfluidic device for the multiplexed electrochemical detection of magneto bioassays. As a proof of concept, the device
CD300f associates with IL-4 receptor α and amplifies IL-4-induced immune cell responses.
Moshkovits I, Karo-Atar D, Itan M, Reichman H, Rozenberg P, Morgenstern-Ben-Baruch N, Shik D, Ejarque-Ortiz A, Hershko AY, Tian L, Coligan JE, Sayós J, Munitz A. Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):8708-13. https://www.ncbi.nlm.nih.gov/pubmed/?term=26124135 Abstract IL-4 receptor (R) α, the common receptor chain for IL-4 and IL-13, is a critical component in
Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A.
Unzueta U, Vázquez F, Accardi G, Mendoza R, Toledo-Rubio V, Giuliani M, Sannino F, Parrilli E, Abasolo I, Schwartz S Jr, Tutino ML, Villaverde A, Corchero JL, Ferrer-Miralles N. Appl Microbiol Biotechnol. 2015 Jul;99(14):5863-7 https://www.ncbi.nlm.nih.gov/pubmed/?term=Strategies+for+the+production+of+difficult-to-express+full-length+eukaryotic+proteins+using+microbial+cell+factories%3A+production+of+human+alpha-galactosidase+A. Abstract Obtaining high levels of pure proteins remains the main bottleneck of many scientific and biotechnological studies. Among all the
Aortic Arch Mycotic Aneurysm Due to Scedosporium Apiospermum Reconstructed With Homografts.
Blasco-Lucas A, Reyes-Juárez JL, Nazarena Pizzi M, Permanyer E, Evangelista A, Galiñanes M. Ann Thorac Surg. 2015 Jun;99(6):2218-20. https://www.ncbi.nlm.nih.gov/pubmed/?term=Aortic+Arch+Mycotic+Aneurysm+Due+to+Scedosporium+Apiospermum+Reconstructed+With+Homografts Abstract A 39-year-old female, active parenteral drug user was diagnosed of spondylodiscitis. A computed tomography (CT) scan showed an extensive aortic arch aneurysm. A positron emission tomography (PET)-CT scan, showing significant aortic wall uptake of the
The Receptor CMRF35-Like Molecule-1 (CLM-1) Enhances the Production of LPS-Induced Pro-Inflammatory Mediators during Microglial Activation.
Ejarque-Ortiz A, Solà C, Martínez-Barriocanal Á, Schwartz S Jr, Martín M, Peluffo H, Sayós J. PLoS One. 2015 Apr 30;10(4):e0123928. doi: 10.1371/journal.pone.0123928. eCollection 2015. https://www.ncbi.nlm.nih.gov/pubmed/?term=The+Receptor+CMRF35-Like+Molecule-1+(CLM-1)+Enhances+the+Production+of+LPS-Induced+Pro-Inflammatory+Mediators+during+Microglial+Activation. Abstract CMRF35-like molecule-1 (CLM-1) belongs to a receptor family mainly expressed in myeloid cells that include activating and inhibitory receptors. CLM-1 contains two ITIMs and a single immunoreceptor tyrosine-based switch
Novel methodology for labelling mesoporous silica nanoparticles using the 18F isotope and their in vivo biodistribution by positron emission tomography
Santiago Rojas Codina; Juan Domingo Gispert; Cristina Menchón; Herme G Baldoví; Mireia Buaki-Sogo; Milagros Rocha; Sergio Abad; Victor Manuel Victor; Hermenegildo Garcia; José Raúl Herance Journal of Nanoparticle Research, March 2015, 17:131 http://link.springer.com/article/10.1007%2Fs11051-015-2938-0 Abstract Nanoparticles have been proposed for several biomedical applications due to their potential as drug carriers, diagnostic and therapeutic agents. However,
Phospholipid profile of amniotic fluid in ovine model of congenital diaphragmatic hernia (CDH): the effect of fetal tracheal occlusion
Mimmi MC, Ballico M, Amoroso F, Calcaterra V, Marotta M, Peiro JL, Pelizzo G. J Proteome Res. 2015 Mar 6;14(3):1465-71. https://www.ncbi.nlm.nih.gov/pubmed/?term=Phospholipid+profile+of+amniotic+fluid+in+ovine+model+of+congenital+diaphragmatic+hernia+(CDH)%3A+the+effect+of+fetal+tracheal+occlusion Abstract Fetal endoscopic tracheal occlusion has been proposed as a prenatal intervention to ameliorate congenital diaphragmatic hernia (CDH) prognosis. Tracheal occlusion (TO) prevents pulmonary fluid egress, leading to tissue expansion, reversal of lung
Duodenal lengthening in short bowel with dilated duodenum.
Bueno J, Redecillas S, García L, Lara A, Giné C, Molino JA, Broto J, Segarra O. J Pediatr Surg. 2015 Mar;50(3):493-6. http://www.sciencedirect.com/science/article/pii/S0022346814007933 Abstract Although duodenal dilatation occurs in children with short bowel syndrome (SBS) facilitating dismotility and bacterial overgrowth, the duodenum has been an untouchable intestinal segment for lengthening procedures owing to its close relationship
Intracellular oxidant activity, antioxidant enzyme defense system, and cell senescence in fibroblasts with trisomy 21.
Rodríguez-Sureda V, Vilches Á, Sánchez O, Audí L, Domínguez C. Oxid Med Cell Longev. 2015;2015:509241. https://www.ncbi.nlm.nih.gov/pubmed/?term=25852816 Abstract Down’s syndrome (DS) is characterized by a complex phenotype associated with chronic oxidative stress and mitochondrial dysfunction. Overexpression of genes on chromosome-21 is thought to underlie the pathogenesis of the major phenotypic features of DS, such as premature
The role of subscapularis muscle denervation in the pathogenesis of shoulder internal rotation contracture after neonatal brachial plexus palsy: a study in a rat model.
Mascarenhas VV, Casaccia M, Fernandez-Martin A, Marotta M, Fontecha CG, Haddad S, Knörr J, Soldado F. J Orthop Res. 2014 Dec;32(12):1675-9. https://www.ncbi.nlm.nih.gov/pubmed/25124991 Abstract We assessed the role of subscapularis muscle denervation in the development of shoulder internal rotation contracture in neonatal brachial plexus injury. Seventeen newborn rats underwent selective denervation of the subscapular muscle. The
RHOA inactivation enhances Wnt signaling and promotes colorectal cancer
Rodrigues P, Macaya I, Bazzocco S, Mazzolini R, Andretta E, Dopeso H, Mateo-Lozano S, Bilić J, Cartón-García F, Nieto R, Suárez-López L, Afonso E, Landolfi S, Hernandez-Losa J, Kobayashi K, Cajal SR, Tabernero J, Tebbutt NC, Mariadason JM, Schwartz S Jr, Arango D. Nature Communications. 2014 Nov 21;5:5458 https://www.ncbi.nlm.nih.gov/pubmed/25413277 Abstract Activation of the small GTPase
Metabolomic profile of amniotic fluid to evaluate lung maturity: the diaphragmatic hernia lamb model.
Pelizzo G, Ballico M, Mimmi MC, Peiró JL, Marotta M, Federico C, Andreatta E, Nakib G, Sampaolesi M, Zambaiti E, Calcaterra V. Multidiscip Respir Med. 2014 Nov 4;9(1):54. https://www.ncbi.nlm.nih.gov/pubmed/?term=Metabolomic+profile+of+amniotic+fluid+to+evaluate+lung+maturity%3A+the+diaphragmatic+hernia+lamb+model. Abstract BACKGROUND: Tracheal occlusion (TO) stimulates lung growth in fetuses affected with congenital diaphragmatic hernia (CDH) although the processes involved in lung maturation still remain unknown.
Electrochemical detection of dopamine using streptavidin-coated magnetic particles and carbon nanotube wiring.
Z Herrasti, F Martínez, E Baldrich Sensors and Actuators B: Chemical. Volume 203, November 2014, Pages 891–898 http://www.sciencedirect.com/science/article/pii/S0925400514008399 Abstract Magnetic particles (MP) and carbon nanotubes (CNT) have been extensively exploited in biosensor development. It has been recently suggested that combining MP binding of electroactive molecules with CNT wiring of the MP surface could provide novel
Prognostic value of plasma chitotriosidase activity in acute stroke patients.
Bustamante A, Dominguez C, Rodriguez-Sureda V, Vilches A, Penalba A, Giralt D, García-Berrocoso T, Llombart V, Flores A, Rubiera M, Molina C, Alvarez-Sabín J, Montaner J. Int J Stroke. 2014 Oct;9(7):910-6. doi: 10.1111/ijs.12160. Epub 2013 Oct 22. Abstract https://www.ncbi.nlm.nih.gov/pubmed/?term=24148838 BACKGROUND AND AIMS: Chitotriosidase, a component of innate immunity, constitutes a sensitive parameter of macrophage activation
Intracellular targeting of CD44+ cells with self-assembling, protein only nanoparticles.
Pesarrodona M, Ferrer-Miralles N; Ugutz Unzueta; Gener P; T Tatkiewivcs; Abasolo I; Ratera I; Veciana J; Schwartz S Jr.; Villaverde A; Vazquez E. Int J Pharmatech. 2014 Oct 1;473(1-2):286-95 https://www.ncbi.nlm.nih.gov/pubmed/?term=Intracellular+targeting+of+CD44%2B+cells+with+self-assembling%2C+protein+only+nanoparticles. Abstract CD44 is a multifunctional cell surface protein involved in proliferation and differentiation, angiogenesis and signaling. The expression of CD44 is up-regulated in several types
Carbon nanotube wiring for signal amplification of electrochemical magneto immunosensors. Application to myeloperoxidase detection.
Z Herrasti, F Martínez, E Baldrich Anal Bioanal Chem, 2014, 406: 5487–5493 https://link.springer.com/article/10.1007/s00216-014-7954-x Abstract In this work, chronoamperometric myelo-peroxidase (MPO) detection was accomplished using immunofunctionalized magnetic microparticles (MPs), disposable carbon screen-printed electrodes (C-SPEs), and a ready-to-use commercially available tetramethylbenzidine (TMB)-based enzymatic substrate. In order to reach the limit of detection (LOD) needed to study real
The role of muscle imbalance in the pathogenesis of shoulder contracture after neonatal brachial plexus palsy: a study in a rat model.
Soldado F, Fontecha CG, Marotta M, Benito D, Casaccia M, Mascarenhas VV, Zlotolow D, Kozin SH. J Shoulder Elbow Surg. 2014 Jul;23(7):1003-9. https://www.ncbi.nlm.nih.gov/pubmed/24388715 Abstract BACKGROUND: An internal rotation contracture of the shoulder is common after neonatal brachial plexus injuries due to subscapularis shortening and atrophy. It has been explained by 2 theories: muscle denervation and
Synthesis of Highly Monodisperse Citrate-Stabilized Silver Nanoparticles of up to 200 nm: Kinetic Control and Catalytic Properties
Neus G. Bastús, Florind Merkoçi Jordi Piella, and Victor Puntes. Chem. Mater., 2014, 26 (9), pp 2836–2846. DOI: 10.1021/cm500316k http://pubs.acs.org/doi/ipdf/10.1021/cm500316k Abstract Highly monodisperse sodium citrate-coated spherical silver nanoparticles (Ag NPs) with controlled sizes ranging from 10 to 200 nm have been synthesized by following a kinetically controlled seeded-growth approach via the reduction of silver nitrate
A new ex vivo method to evaluate the performance of candidate MRI contrast agents: a proof-of-concept study.
Candiota AP, Acosta M, Simões RV, Delgado-Goñi T, Lope-Piedrafita S, Irure A, Marradi M, Bomati-Miguel O, Miguel-Sancho N, Abasolo I, Schwartz S Jr, Santamaria J, Penadés S, Arús C. J Nanobiotechnology. 2014 Apr 5;12:12. https://www.ncbi.nlm.nih.gov/pubmed/?term=A+new+ex+vivo+method+to+evaluate+the+performance+of+candidate+MRI+contrast+agents%3A+a+proof-of-concept+study. Abstract BACKGROUND: Magnetic resonance imaging (MRI) plays an important role in tumor detection/diagnosis. The use of exogenous contrast agents (CAs)
Synthesis of Highly Monodisperse Citrate-Stabilized Silver Nanoparticles of up to 200 nm: Kinetic Control and Catalytic Properties
Neus G. Bastús, Florind Merkoçi, Jordi Piella, and Victor Puntes Chem. Mater., 2014, 26 (9), pp 2836–2846 http://pubs.acs.org/doi/abs/10.1021/cm500316k Abstract Highly monodisperse sodium citrate-coated spherical silver nanoparticles (Ag NPs) with controlled sizes ranging from 10 to 200 nm have been synthesized by following a kinetically controlled seeded-growth approach via the reduction of silver nitrate by the
Electrochemical biosensing of non-electroactive targets using ferrocene-labeled magnetic particles and CNT wiring
Z Herrasti, R Olivé-Monllau, FX Muñoz-Pascual, F Martínez, E Baldrich Analyst. 2014 Mar 21;139(6):1334-9. https://www.ncbi.nlm.nih.gov/pubmed/?term=Electrochemical+biosensing+of+non-electroactive+targets+using+ferrocene-labeled+magnetic+particles+and+CNT+wiring Abstract We show that target binding onto ferrocene-modified magnetic microparticles (MP-Fc) promotes physical sheltering of the labels. This can be measured electrochemically by CNT wiring, which enhances ten-fold the signals registered compared to direct detection of the MPs alone. As
HAVCR/KIM-1 activates the IL-6/STAT-3 pathway in clear cell renal cell carcinoma and determines tumor progression and patient outcome.
Cuadros T, Trilla E, Sarró E, Vilà MR, Vilardell J, de Torres I, Salcedo M, López-Hellin J, Sánchez A, Ramón y Cajal S, Itarte E, Morote J, Meseguer A. Cancer Res. 2014 Mar 1;74(5):1416-28. https://www.ncbi.nlm.nih.gov/pubmed/?term=HAVCR%2FKIM-1+activates+the+IL-6%2FSTAT-3+Pathway+in+ccRCC+and+Determines+Tumor+Progression+and+Patient+Outcome Abstract Renal cell carcinoma (RCC), the third most prevalent urological cancer, claims more than 100,000 lives/year worldwide. The clear cell
Maternal and foetal angiogenic imbalance in congenital heart defects
Llurba E, Sánchez O, Ferrer Q, Nicolaides KH, Ruíz A, Domínguez C, Sánchez-de-Toledo J, García-García B, Soro G, Arévalo S, Goya M, Suy A, Eur Heart J. 2014 Mar;35(11):701-7Pérez-Hoyos S, Alijotas-Reig J, Carreras E, Cabero L. https://www.ncbi.nlm.nih.gov/pubmed/?term=24159191 Abstract AIMS: Animal models showed that angiogenesis is related to abnormal heart development. Our objectives were to ascertain
Fluorescent molecular peroxidation products: a prognostic biomarker of early neurologic deterioration after thrombolysis.
Llombart V, Dominguez C, Bustamante A, Rodriguez-Sureda V, Martín-Gallán P, Vilches A, García-Berrocoso T, Penalba A, Hernández-Guillamon M, Rubiera M, Ribó M, Eschenfelder C, Giralt D, Molina CA, Alvarez-Sabín J, Rosell A, Montaner J. Stroke. 2014 Feb;45(2):432-7. https://www.ncbi.nlm.nih.gov/pubmed/?term=24335228 Abstract BACKGROUND AND PURPOSE: Fluorescent molecular peroxidation products (FMPPs) are considered potential markers of molecular oxidative damage
Effect of the transducer’s surface pre-treatment on SPR aptasensor development.
P Rodríguez-Franco, Ll Abad, FX Muñoz-Pascual, M Moreno, E Baldrich Sens Act B, (2014) Volume 191, February 2014, Pages 634–642 http://dx.doi.org/10.1016/j.snb.2013.10.046 Abstract The properties of a transducer’s surface are crucial in biosensor development since they strongly condition the chemical routes applicable for bioreceptor incorporation and subsequent surface physical blocking. In this way, surface (bio)engineering should
Can simulation help to answer the demand for echocardiography education?
Clau-Terré F, Sharma V, Cholley B, Gonzalez-Alujas T, Galiñanes M, Evangelista A, Fletcher N. Anesthesiology. 2014 Jan;120(1):32-41. https://www.ncbi.nlm.nih.gov/pubmed/?term=3.%09Can+simulation+help+to+answer+the+demand+for+echocardiography+education%3F Abstract There has been a recent explosion of education and training in echocardiography in the specialties of anesthesiology and critical care. These devices, by their impact on clinical management, are changing the way surgery is performed and
Single-Access Foetal Endoscopy (SAFE) for myelomeningocele in sheep model I: amniotic carbon dioxide gas approach.
Peiro JL, Fontecha CG, Ruano R, Esteves M, Fonseca C, Marotta M, Haeri S, Belfort MA. Surg Endosc. 2013; 27(10):3835-40. https://www.ncbi.nlm.nih.gov/pubmed/23670742 Abstract BACKGROUND: This study aimed to assess the feasibility of single-access fetal endoscopy (SAFE) for the management of myelomeningocele (MMC) using intrauterine carbon dioxide as a distension medium in a sheep model. METHODS: This
Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform
Gomes-da-Silva LC, Fernández Y, Abasolo I, Schwartz S Jr, Ramalho JS, Pedroso de Lima MC, Simões S, Moreira JN. Nanomedicine (Lond). 2013 Sep;8(9):1397-413. http://www.futuremedicine.com/doi/abs/10.2217/nnm.12.174 Abstract AIM: The design of novel F3-targeted liposomes with adequate features for systemic administration, to enable efficient intracellular delivery of siRNA toward both cancer and endothelial cells from angiogenic blood vessels.
Chronoamperometric MagnetoImmunosensor for Myeloperoxidase Detection in Human Plasma Based on a Magnetic Switch Produced by 3D Laser Sintering
J Barallat, R Olivé-Monllau, J Gonzalo-Ruiz, R Ramírez-Satorras, FX Muñoz-Pascual, A Galán Ortega, E Baldrich Anal Chem, 2013, 85 (19), pp 9049–9056 http://pubs.acs.org/doi/abs/10.1021/ac401549d Abstract In this work, an amperometric immunosensor for detection of myeloperoxidase (MPO) in human plasma is reported. Detection is based on the immobilization of anti-MPO antibodies onto magnetic beads (MBs). Following MPO
Multifunctional Nanovesicle-Bioactive Conjugates Prepared by a One-Step Scalable Method Using CO2-Expanded Solvents
Cabrera I, Elizondo E, Esteban O, Corchero JL, Melgarejo M, Pulido D, Cordoba A, Moreno E, Unzueta U, Vazquez E, Abasolo I, Schwartz S Jr, Villaverde A, Albericio F, Royo M, Garcia-Parajo MF, Ventosa N, Veciana J. Nano Lett. 2013 Aug 14;13(8):3766-74 http://pubs.acs.org/doi/abs/10.1021/nl4017072
Erythrocytes labeled with [(18) F]SFB as an alternative to radioactive CO for quantification of blood volume with PET.
Herance JR, Gispert JD, Abad S, Victor VM, Pareto D, Torrent È, Rojas S. Contrast Media Mol Imaging.8 – 4, pp. 375 – 381. 01/07/2013 https://www.ncbi.nlm.nih.gov/pubmed/?term=Erythrocytes+labeled+with+%5B(18)+F%5DSFB+as+an+alternative+to+radioactive+CO+for+quantification+of Abstract Inhaled radioactive CO is currently the tracer of choice for blood volume quantification by positron emission tomography (PET). This measurement is of great interest for several clinical and
Semisynthesis, Cytotoxic Activity, and Oral Availability of New Lipophilic 9-Substituted Camptothecin Derivatives
Rodriguez-Berna, G ; Cabanas, MJD ; Mangas-Sanjuan, V ; Gonzalez-Alvarez, M ; Gonzalez-Alvarez, I ; Abasolo, I ; Schwartz S Jr ; Bermejo, M; Corma, A ACS Medicinal Chemistry Letters, Volume: 4 Issue: 7 Pages: 86-90. May 28, 2013 http://pubs.acs.org/doi/abs/10.1021/ml400125z Abstract Despite that 9-substituted camptothecins are promising candidates in cancer therapy, the limited accessibility to this position
Hepatitis A virus cellular receptor 1/kidney injury molecule-1 is a susceptibility gene for clear cell renal cell carcinoma and hepatitis A virus cellular receptor/kidney injury molecule-1 ectodomain shedding a predictive biomarker of tumour progression.
Cuadros T, Trilla E, Vilà MR, de Torres I, Vilardell J, Messaoud NB, Salcedo M, Sarró E, López-Hellin J, Blanco A, Mir C, Ramón y Cajal S, Itarte E, Morote J, Meseguer A. Eur J Cancer. 2013 May;49(8):2034-47. doi: 10.1016/j.ejca.2012.12.020. Epub 2013 Jan 23. https://www.ncbi.nlm.nih.gov/pubmed/?term=Hepatitis+A+virus+cellular+receptor+1%2Fkidney+injury+molecule-1+is+a+susceptibility+gene+for+clear+cell+renal+cell+carcinoma+and+hepatitis+A+virus+cellular+receptor%2Fkidney+injury+molecule-1+ectodomain+shedding+a+predictive+biomarker+of+tumour+progression Abstract AIM OF THE STUDY: To correlate hepatitis A virus
Brush border myosin Ia inactivation in gastric but not endometrial tumors.
Mazzolini R, Rodrigues P, Bazzocco S, Dopeso H, Ferreira AM, Mateo-Lozano S, Andretta E, Woerner SM, Alazzouzi H, Landolfi S, Hernandez-Losa J, Macaya I, Suzuki H, Cajal SR, Mooseker MS, Mariadason JM, Gebert J, Hofstra RM, Reventós J, Yamamoto H, Jr SS, Arango D. Int J Cancer. 2013 Apr 15;132(8):1790-9. https://www.ncbi.nlm.nih.gov/pubmed/23002058 Abstract Brush border Myosin
Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics
Corchero J.L., Gasser B., Resina D., Smith W., Parrilli E., Vázquez F., Abasolo I., Giuliani M., Jäntti J., Ferrer P., Saloheimo M., Mattanovich D., Schwartz S. Jr, Tutino L., Villaverde A. Biotechnology Advances. 2013 Mar; 31(2):140-53. https://www.ncbi.nlm.nih.gov/pubmed/22985698 Abstract Both conventional and innovative biomedical approaches require cost-effective protein drugs with high therapeutic potency, improved bioavailability, biocompatibility,
A form of apolipoprotein a-I is found specifically in relapses of focal segmental glomerulosclerosis following transplantation.
Lopez-Hellin J, Cantarell C, Jimeno L, Sanchez-Fructuoso A, Puig-Gay N, Guirado L, Vilariño N, Gonzalez-Roncero FM, Mazuecos A, Lauzurica R, Burgos D, Plumed JS, Jacobs-Cacha C, Jimenez C, Fernandez A, Fernandez-Alvarez P, Torregrosa V, Nieto JL, Meseguer A, Alonso A; GREAT Study Group. Am J Transplant. 2013 Feb;13(2):493-500. doi: 10.1111/j.1600-6143.2012.04338.x. Epub 2012 Dec 3. https://www.ncbi.nlm.nih.gov/pubmed/?term=A+form+of+apolipoprotein+A-I+is+found+specifically+in+relapses+of+focal+segmental+glomerulosclerosis+following+transplantation
Iridium Oxide pH sensor for biomedical applications. Case urea-urease in real urine samples.
E Prats-Alfonso, Ll Abad, N Casan-Pastor, J Gonzalo-Ruiz, E Baldrich Biosens Bioelectron. 2013 Jan 15;39(1):163-9 http://www.sciencedirect.com/science/article/pii/S0956566312004629 Abstract This work demonstrates the implementation of iridium oxide films (IROF) grown on silicon-based thin-film platinum microelectrodes, their utilization as a pH sensor, and their successful formatting into a urea pH sensor. In this context, Pt electrodes were fabricated
Differences in performance of five types of aortic valve prostheses: haemodynamic assessment by dobutamine stress echocardiography.
Khoo JP, Davies JE, Ang KL, Galiñanes M, Chin DT. Heart. 2013 Jan;99(1):41-7 https://www.ncbi.nlm.nih.gov/pubmed/?term=Differences+in+performance+of+five+types+of+aortic+valve+prostheses%3A+haemodynamic+assessment+by+dobutamine+stress+echocardiography. Abstract BACKGROUND: In patients being considered for aortic valve replacement, there remains controversy over which design or tissue offers the best performance. We aimed to evaluate in a single study the haemodynamic performances of five different widely used aortic valve prostheses:
In vivo biodistribution of amino-functionalized ceria nanoparticles in rats using positron emission tomography.
Rojas S, Gispert JD, Abad S, Buaki-Sogo M, Victor VM, Garcia H, Herance JR. Mol Pharm. 2012 Dec 3;9(12):3543-50. doi: 10.1021/mp300382n. Epub 2012 Nov 20. https://www.ncbi.nlm.nih.gov/pubmed/?term=In+Vivo+Biodistribution+of+Amino-Functionalized+Ceria+Nanoparticles+in+Rats+Using Abstract A variety of nanoparticles have been proposed for several biomedical applications. To gauge the therapeutic potential of these nanoparticles, in vivo biodistribution is essential and mandatory. In the
Immediate and long-term outcome of left-sided infective endocarditis. A 12-year prospective study from a contemporary cohort in a referral hospital.
Fernández-Hidalgo N1, Almirante B, Tornos P, González-Alujas MT, Planes AM, Galiñanes M, Pahissa A. Clin Microbiol Infect. 2012 Dec;18(12):E522-30. https://www.ncbi.nlm.nih.gov/pubmed/?term=5.%09Immediate+and+long-term+outcome+of+left-sided+infective+endocarditis.+A+12-year+prospective+study+from+a+contemporary+cohort+in+a+referral+hospital. Abstract The aim of this study was to describe the immediate and long-term prognosis of a contemporary cohort of patients with left-sided infective endocarditis (LSIE). A prospective observational cohort study was conducted in a referral
Gold nanoparticles supported on nanoparticulate ceria as a powerful agent against intracellular oxidative stress.
Menchón C, Martín R, Apostolova N, Victor VM, Alvaro M, Herance JR, García H. Small. 2012 Jun 25;8(12):1895-903. https://www.ncbi.nlm.nih.gov/pubmed/?term=Gold+Nanoparticles+Supported+on+Nanoparticulate+Ceria+as+a+Powerful+Agent+against Abstract Ceria–supported gold nanoparticles are prepared exhibiting peroxidase activity and acting as radical traps. Au/CeO(2) shows a remarkable biocompatibility as demonstrated by measuring cellular viability, proliferation, and lack of apoptosis for two human cell lines (Hep3B
Rapid and high-yielding cysteine labelling of peptides with N-succinimidyl 4-[18F]fluorobenzoate.
Abad S, Nolis P, Gispert JD, Spengler J, Albericio F, Rojas S, Herance JR. Chem Commun (Camb). 2012 Jun 21;48(49):6118-20 https://www.ncbi.nlm.nih.gov/pubmed/?term=Rapid+and+high-yielding+cysteine+labelling+of+peptides+with+N-succinimidyl Abstract Fast cysteine labelling of peptides promoted by an adjacent arginine has been observed with a standard labelling agent specific for amines, N-succinimidyl 4-[(18)F]fluorobenzoate.
Beta-catenin confers resistance to PI3K and AKT inhibitors and subverts FOXO3a to promote metastasis in colon cancer
Tenbaum SP, Ordóñez-Morán P, Puig I, Chicote I, Arqués O, Landolfi S, Fernández Y, Herance JR, Gispert JD, Mendizabal L, Aguilar S, Cajal SR, Schwartz S Jr, Vivancos A, Espín E, Rojas S, Baselga J, Tabernero J, Muñoz A, Palmer HG. Nat Med., 2012 Jun;18(6):892-901 https://www.ncbi.nlm.nih.gov/pubmed/22610277 Abstract The Wnt–β-catenin and PI3K-AKT-FOXO3a pathways have a central
Overexpression of the immunoreceptor CD300f has a neuroprotective role in a model of acute brain injury.
Peluffo H, Alí-Ruiz D, Ejarque-Ortíz A, Heras-Alvarez V, Comas-Casellas E, Martínez-Barriocanal A, Kamaid A, Alvarez-Errico D, Negro ML, Lago N, Schwartz S Jr, Villaverde A, Sayós J. Brain Pathol. 2012 May;22(3):318-28. https://www.ncbi.nlm.nih.gov/pubmed/?term=Overexpression+of+the+immunoreceptor+CD300f+has+a+neuroprotective+role+in+a+model+of+acute+brain+injury. Abstract It is well known that cell surface immune receptors play a critical role in regulating immune and inflammatory processes in the central
Vascularized fibular periosteal graft: a new technique to enhance bone union in children.
Soldado F, Fontecha CG, Barber I, Velez R, Llusa M, Collado D, Rodriguez-Baeza A, Martinez-Ibañez V. J Pediatr Orthop. 2012 Apr-May;32(3):308-13. https://www.ncbi.nlm.nih.gov/pubmed/22411339 Abstract BACKGROUND: The periosteum in children has strong osteogenic power and is quite thick, facilitating procurement. However, it has been rarely used as a vascularized flap to enhance bone union in this age
Villin expression is frequently lost in poorly differentiated colon cancer.
Arango D, Al-Obaidi S, Williams DS, Dopeso H, Mazzolini R, Corner G, Byun DS, Carr AA, Murone C, Tögel L, Zeps N, Aaltonen LA, Iacopetta B, Mariadason JM. Am J Pathol. 2012 Apr;180(4):1509-21 https://www.ncbi.nlm.nih.gov/pubmed/22349300 Abstract Colorectal cancers (CRCs) are classified as having microsatellite instability (MSI) or chromosomal instability (CIN); herein termed microsatellite stable (MSS). MSI
Cloning and characterization of CD300d, a novel member of the human CD300 family of immune receptors.
Comas-Casellas E, Martínez-Barriocanal Á, Miró F, Ejarque-Ortiz A, Schwartz S Jr, Martín M, Sayós J. J Biol Chem. 2012 Mar 23;287(13):9682-93. https://www.ncbi.nlm.nih.gov/pubmed/?term=22291008 Abstract Herein we present the cloning and molecular characterization of CD300d, a member of the human CD300 family of immune receptors. CD300d cDNA was cloned from RNA obtained from human peripheral blood
First trimester serum angiogenic/anti-angiogenic status in twin pregnancies: relationship with assisted reproduction technology.
Sánchez O, Llurba E, Marsal G, Domínguez C, Aulesa C, Sánchez-Durán MA, Goya MM, Alijotas-Reig J, Carreras E, Cabero L. Hum Reprod. 2012 Feb;27(2):358-65. https://www.ncbi.nlm.nih.gov/pubmed/?term=22116949 Abstract BACKGROUND: The risk of pre-eclampsia (PE) increases in twin pregnancies, especially when assisted reproduction technologies (ART) are used. The aim of this study was to assess angiogenic/anti-angiogenic factors in
Brush border Myosin Ia has tumor suppressor activity in the intestine.
Mazzolini R, Dopeso H, Mateo-Lozano S, Chang W, Rodrigues P, Bazzocco S, Alazzouzi H, Landolfi S, Hernández-Losa J, Andretta E, Alhopuro P, Espín E, Armengol M, Tabernero J, Ramón Y Cajal S, Kloor M, Gebert J, Mariadason JM, Schwartz S Jr, Aaltonen LA, Mooseker MS, Arango D. Proc Natl Acad Sci U S A. 2012
Synthesis and In Vivo Evaluation of the Biodistribution of a F-18-Labeled Conjugate Gold-Nanoparticle-Peptide with Potential Biomedical Application
Simon Guerrero; Jose Raul Herance; Santiago Rojas; Juan F. Mena; Juan Domingo Gispert; Gerardo A. Acosta; Fernando Albericio; Marcelo J. Kogan. BIOCONJUGATE CHEMISTRY. 23 -3, pp. 399 – 408. 03/2012. http://repositorio.uchile.cl/bitstream/handle/2250/121717/Guerrero_Simon.pdf;sequence=1 ABSTRACT Gold nanoparticles (AuNPs) have been extensively used in biological applications because of their biocompatibility, size, and ease of characterization, as well as an
A pharmacologically-based array to identify targets of cyclosporine A-induced toxicity in cultured renal proximal tubule cells.
Sarró E, Jacobs-Cachá C, Itarte E, Meseguer A. Toxicol Appl Pharmacol. 2012 Jan 15;258(2):275-87. doi: 10.1016/j.taap.2011.11.007. Epub 2011 Dec 3. https://www.ncbi.nlm.nih.gov/pubmed/?term=A+pharmacologically-based+array+to+identify+targets+of+cyclosporine+A-induced+toxicity+in+cultured+renal+proximal+tubule+cells Abstract Mechanisms of cyclosporine A (CsA)-induced nephrotoxicity were generally thought to be hemodynamic in origin; however, there is now accumulating evidence of a direct tubular effect. Although genomic and proteomic experiments by our
Study of biomaterial-induced macrophage activation, cell-mediated immune response and molecular oxidative damage in patients with dermal bioimplants.
Sánchez O, Rodríguez-Sureda V, Domínguez C, Fernández-Figueras T, Vilches A, Llurba E, Alijotas-Reig J. Immunobiology. 2012 Jan;217(1):44-53. doi: 10.1016/j.imbio.2011.08.003. Epub 2011 Aug 12. https://www.ncbi.nlm.nih.gov/pubmed/?term=21903293 Abstract Several soft-tissue dermal fillers have been reported to provoke immunogenicity and may cause adverse reactions despite claims regarding their safety. This study aimed to assess biomaterial-induced macrophage activation, cell-mediated immune
Hardening of the nanoparticle-protein corona in metal (Au, Ag) and oxide (Fe3O4, CoO, and CeO2) nanoparticles.
Casals E, Pfaller T, Duschl A, Oostingh GJ, Puntes VF. Small. 2011 Dec 16;7(24):3479-86. doi: 10.1002/smll.201101511. Epub 2011 Nov 7. https://www.ncbi.nlm.nih.gov/pubmed/?term=Hardening+of+the+nanoparticle%E2%80%93protein+corona+in+metal+(Au%2C+Ag)+and+oxide+(Fe3O4%2C+CoO%2C+and+CeO2)+nanoparticles Abstract The surface modifications of metal and metal oxide nanoparticles with sizes ranging from 7 to 20 nm dispersed in commonly used cell culture medium supplemented with serum are investigated. All the tested
Surface-modified silica nanoparticles for tumor-targeted delivery of camptothecin and its biological evaluation
Botella P, Abasolo I, Fernández Y, Muniesa C, Miranda S, Quesada M, Ruiz J, Schwartz S Jr, Corma A. J Control Release. 2011 Dec 10;156(2):246-57 http://www.sciencedirect.com/science/article/pii/S0168365911004743 Abstract Here we report the design, synthesis and biological evaluation of surface-modified silica nanoparticles (SNP) for the delivery of camptothecin (CPT). Drug has been covalently linked to the nanoparticle
Carving at the nanoscale: sequential galvanic exchange and Kirkendall growth at room temperature
González E, Arbiol J, Puntes VF. Science. 2011 Dec 9;334(6061):1377-80 https://www.ncbi.nlm.nih.gov/pubmed/?term=Carving+at+the+nanoscale%3A+sequential+galvanic+exchange+and+Kirkendall+growth+at+room+temperature Abstract Shape control of inorganic nanocrystals is important for understanding basic size- and shape-dependent scaling laws and is useful in a wide range of applications. With minor modifications in the chemical environment, it is possible to control the reaction and diffusion processes at
Positron emission tomographic imaging of the cannabinoid type 1 receptor system with [¹¹C]OMAR ([¹¹C]JHU75528): improvements in image quantification using wild-type and knockout mice.
Herance R, Rojas S, Abad S, Jiménez X, Gispert JD, Millán O, Martín-García E, Burokas A, Serra MÀ, Maldonado R, Pareto D. Mol Imaging. 2011 Dec;10(6):481-7. https://www.ncbi.nlm.nih.gov/pubmed/22201539 Abstract In this study, we assessed the feasibility of using positron emission tomography (PET) and the tracer [¹¹C]OMAR ([¹¹C]JHU75528), an analogue of rimonabant, to study the brain cannabinoid
Changes in oxidant-antioxidant status in young diabetic patients from clinical onset onwards.
Martín-Gallán P, Carrascosa A, Gussinyé M, Domínguez C. J Cell Mol Med. 2007 Nov-Dec;11(6):1352-66. doi: 10.1111/j.1582-4934.2007.00068.x. https://www.ncbi.nlm.nih.gov/pubmed/?term=18205705 Abstract Oxidative stress has been implicated as a mechanism underlying hyperglycaemia-associated cellular damage and could play a role in the development of diabetes-related complications. This study aimed to evaluate the significance of changes in oxidant-antioxidant status in 176
Increased oxidative stress, the renin-angiotensin system, and sympathetic overactivation induce hypertension in kidney androgen-regulated protein transgenic mice.
Grande MT, Pascual G, Riolobos AS, Clemente-Lorenzo M, Bardaji B, Barreiro L, Tornavaca O, Meseguer A, López-Novoa JM. Free Radic Biol Med. 2011 Nov 15;51(10):1831-41. https://www.ncbi.nlm.nih.gov/pubmed/?term=Increased+oxidative+stress%2C+renin-angiotensin+system+and+sympathetic+overactivation+induce+hypertension+in+kidney+androgen-regulated+protein+transgenic+mice Abstract Gender differences in the incidence and severity of hypertension have suggested the involvement of a sex-dependent mechanism. Transgenic (Tg) mice overexpressing kidney androgen-regulated protein (KAP) specifically in kidney
Carbon nanotube wiring: a tool for straightforward electrochemical biosensing at magnetic particles
E Baldrich, FX Muñoz Anal. Chem., 2011, 83 (24), pp 9244–9250 http://pubs.acs.org/doi/abs/10.1021/ac201137q Abstract Here, we combine the unique properties of carbon nanotubes (CNTs) and magnetic particles (MPs) to develop a novel biosensing approach for the specific detection of electroactive labels and targets. The assay is based on label/target capture and concentration using MPs. It follows
KAP degradation by calpain is associated with CK2 phosphorylation and provides a novel mechanism for cyclosporine A-induced proximal tubule injury.
Tornavaca O, Sarró E, Pascual G, Bardaji B, Montero MA, Salcedo MT, Plana M, López-Hellin J, Itarte E, Meseguer A. PLoS One. 2011;6(9):e25746. doi: 10.1371/journal.pone.0025746. Epub 2011 Sep 28. https://www.ncbi.nlm.nih.gov/pubmed/?term=KAP+degradation+by+calpain+associates+with+CK2+phosphorylation+and+provides+a+novel+mechanism+for+Cyclosporine+A-induced+kidney+toxicity Abstract The use of cyclosporine A (CsA) is limited by its severe nephrotoxicity that includes reversible vasoconstrictor effects and proximal tubule cell injury, the latter
Endogenous cardiac stem cell activation by insulin-like growth factor-1/hepatocyte growth factor intracoronary injection fosters survival and regeneration of the infarcted pig heart.
Ellison GM, Torella D, Dellegrottaglie S, Perez-Martinez C, Perez de Prado A, Vicinanza C, Purushothaman S, Galuppo V, Iaconetti C, Waring CD, Smith A, Torella M, Cuellas Ramon C, Gonzalo-Orden JM, Agosti V, Indolfi C, Galiñanes M, Fernandez-Vazquez F, Nadal-Ginard B. J Am Coll Cardiol. 2011 Aug 23;58(9):977-86. https://www.ncbi.nlm.nih.gov/pubmed/?term=Endogenous+cardiac+stem+cell+activation+by+insulin-like+growth+factor-1%2Fhepatocyte+growth+factor+intracoronary+injection+fosters+survival+and+regeneration+of+the+infarcted+pig+heart Abstract OBJECTIVES: The purpose of this
Biodistribution of amino-functionalized diamond nanoparticles. In vivo studies based on 18F radionuclide emission.
Rojas S, Gispert JD, Martín R, Abad S, Menchón C, Pareto D, Víctor VM, Alvaro M, García H, Herance JR. ACS Nano. 2011 Jul 26;5(7):5552-9. https://www.ncbi.nlm.nih.gov/pubmed/?term=Biodistribution+of+Amino-Functionalized+Diamond Abstract Nanoparticles have been proposed for several biomedical applications; however, in vivo biodistribution studies to confirm their potential are scarce. Nanodiamonds are carbon nanoparticles that have been recently proposed
Kinetically controlled seeded growth synthesis of citrate-stabilized gold nanoparticles of up to 200 nm: size focusing versus Ostwald ripening
Neus G. Bastús, Joan Comenge, and Víctor Puntes Langmuir, 2011, 27 (17), pp 11098–11105 http://pubs.acs.org/doi/abs/10.1021/la201938u Abstract Monodisperse citrate-stabilized gold nanoparticles with a uniform quasi-spherical shape of up to ∼200 nm and a narrow size distribution were synthesized following a kinetically controlled seeded growth strategy via the reduction of HAuCl4 by sodium citrate. The inhibition of
Electrochemical Detection of Testosterone by Use of Three-Dimensional Disc-Ring Microelectrode Sensing Platforms: Application to Doping Monitoring
O Laczka, J del Campo, FX Muñoz-Pascual, E Baldrich Analytical Chemistry 83(11):4037-44 · June 2011 https://www.researchgate.net/publication/51053729_Electrochemical_Detection_of_Testosterone_by_Use_of_Three-Dimensional_Disc-Ring_Microelectrode_Sensing_Platforms_Application_to_Doping_Monitoring Abstract Testosterone is one of the androgenic steroid hormones, the consumption of which is considered doping in most sports. Here, we present powerful 3D sensing platforms using novel disc-ring microelectrode array devices and exploit them for the competitive immunosensing of
Fetoscopic coverage of experimental myelomeningocele in sheep using a patch with surgical sealant.
Fontecha CG, Peiro JL, Sevilla JJ, Aguirre M, Soldado F, Fresno L, Fonseca C, Chacaltana A, Martinez V. Eur J Obstet Gynecol Reprod Biol. 2011 Jun;156(2):171-6. https://www.ncbi.nlm.nih.gov/pubmed/21353374 Abstract OBJECTIVE: Assess the feasibility of a fetoscopic patch coverage method for myelomeningocele repair in a sheep model. STUDY DESIGN: Experimental study. A myelomeningocele-like defect was created in
Improved bacteria detection by coupling magneto-immunocapture and amperometry at flow-channel microband electrodes
O Laczka, JM Maesa, N Godino, J del Campo, M Fougt-Hansen, JP Kutter, D Snakenborg, FX Muñoz-Pascual, E Baldrich Biosens Bioelectron. 2011 Apr 15;26(8):3633-40 https://www.ncbi.nlm.nih.gov/pubmed/21392960
Acute toxicity of cerium oxide, titanium oxide and iron oxide nanoparticles using standardized tests
A García, R Espinosa, L Delgado, E Casals, E González, V Puntes, C Barata, X Font, A Sánchez Desalination, Volume 269, Issues 1–3, 15 March 2011, Pages 136–141 http://www.sciencedirect.com/science/article/pii/S0011916410007678 Abstract In this study, several standard toxicity tests have been performed on selected inorganic nanoparticles. Acute toxicity tests were selected according to their extensive use in toxicological
Magnetic entrapment as a fast and simple strategy for electrode modification with carbon nanotubes. Application to dopamine detection
E Baldrich, R Gómez, G Gabriel, FX Muñoz Biosens Bioelectron. 2011 Jan 15;26(5):1876-82 https://www.ncbi.nlm.nih.gov/pubmed/20378329 Abstract Carbon nanotubes (CNT) have been exploited for an important number of electroanalytical and sensing purposes. Specifically, CNT incorporation to an electrode surface coating increases its roughness and area, provides electrocatalytic activity towards a variety of molecules, and improves electron transfer.
High EPHB2 mutation rate in gastric but not endometrial tumors with microsatellite instability.
Davalos v, Dopeso H, Velho S, Monteiro Ferreira A, Cirnes L, Díaz-Chico N, Bilbao C, Ramírez R, Rodríguez G, Falcón O, León L, Niessen R, Keller G, Dallenbach-Hellweg G, Espín E, Armengol M, Perucho M, Imai K, Yamamoto H, Gebert JF, Díaz-Chico JC, Hofstra RM, Woerner SM, Seruca R, Schwartz Jr. S, Arango D. Oncogene. 2007
CD300 heterocomplexes, a new and family-restricted mechanism for myeloid cell signaling regulation.
Martínez-Barriocanal A, Comas-Casellas E, Schwartz S Jr, Martín M, Sayós J. J Biol Chem. 2010 Dec 31;285(53):41781-94. https://www.ncbi.nlm.nih.gov/pubmed/?term=20959446 Abstract The CD300 family of myeloid immunoglobulin receptors includes activating (CD300b, CD300e) and inhibitory members (CD300a, CD300f), as well as molecules of uncertain function presenting a negative charge within their transmembrane domain (CD300c, CD300d). In this paper,
Nano-jewels in biology. Gold and platinum on diamond nanoparticles as antioxidant systems against cellular oxidative stress.
Martín R, Menchón C, Apostolova N, Victor VM, Alvaro M, Herance JR, García H. ACS Nano. 2010 Nov 23;4(11):6957-65. https://www.ncbi.nlm.nih.gov/pubmed/?term=Gold+and+Platinum+on+Diamond+Nanoparticles+as+Antioxidant Abstract Diamond nanoparticles (DNPs) obtained by explosive detonation have become commercially available. These commercial DNPs can be treated under Fenton conditions (FeSO(4) and H(2)O(2) at acidic pH) to obtain purer DNP samples with a small
Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney.
Suñé G, Sarró E, Puigmulé M, López-Hellín J, Zufferey M, Pertel T, Luban J, Meseguer A. PLoS One. 2010 Nov 10;5(11):e13930. https://www.ncbi.nlm.nih.gov/pubmed/?term=Cyclophilin+B+interacts+with+sodium-potassium+ATPase+and+is+required+for+pump+activity+in+proximal+tubule+cells+of+the+kidney Abstract Cyclophilins (Cyps), the intracellular receptors for Cyclosporine A (CsA), are responsible for peptidyl-prolyl cis-trans isomerisation and for chaperoning several membrane proteins. Those functions are inhibited upon CsA binding. Albeit its great
Correlating physico-chemical with toxicological properties of nanoparticles: the present and the future.
Rivera Gil P, Oberdörster G, Elder A, Puntes V, Parak WJ. ACS Nano. 2010 Oct 26;4(10):5527-31. https://www.ncbi.nlm.nih.gov/pubmed/?term=Correlating+physico-chemical+with+toxicological+properties+of+nanoparticles%3A+the+present+and+the+future Abstract Nanotoxicology is still a new discipline. In this Perspective, both its origins and its future trends are discussed. In particular, we note several issues we consider important for publications in this field.
A genetic defect in exportin-5 traps precursor microRNAs in the nucleus of cancer cells
Melo SA, Moutinho C, Ropero S, Calin GA, Rossi S, Spizzo R, Fernandez AF, Davalos V, Villanueva A, Montoya G, Yamamoto H, Schwartz S Jr, Esteller M. Cancer Cell. 2010 Oct 19;18(4):303-15. http://www.cell.com/cancer-cell/abstract/S1535-6108(10)00344-2 Abstract The global impairment of mature microRNAs (miRNAs) is emerging as a common feature of human tumors. One interesting scenario is that
Amperometric detection of Enterobacteriacae by measuring β-galactosidase activity at interdigitated microelectrode arrays
O Laczka, C García-Aljaro, FJ del Campo, FX Muñoz, J Mas, E Baldrich Anal Chim Acta. 2010 Sep 16;677(2):156-61 https://www.ncbi.nlm.nih.gov/pubmed/20837182 Abstract Two simple methodologies are compared for the detection of faecal contamination in water using amperometry at gold interdigitated microelectrodes. They rely on the detection of β-galactosidase (β-gal) by redox cycling amperometry of the p-aminophenol
Time evolution of the nanoparticle protein corona
Casals E, Pfaller T, Duschl A, Oostingh GJ, Puntes V. ACS Nano. 2010 Jul 27;4(7):3623-32. https://www.ncbi.nlm.nih.gov/pubmed/20553005 Abstract In this work, we explore the formation of the protein corona after exposure of metallic Au nanoparticles (NPs), with sizes ranging from 4 to 40 nm, to cell culture media containing 10% of fetal bovine serum. Under in
Coordination polymer particles as potential drug delivery systems.
Imaz I, Rubio-Martínez M, García-Fernández L, García F, Ruiz-Molina D, Hernando J, Puntes V, Maspoch D. Chem Commun (Camb). 2010 Jul 14;46(26):4737-9. doi: 10.1039/c003084h. Epub 2010 May 20. https://www.ncbi.nlm.nih.gov/pubmed/20485835 Abstract Micro- and nanoscale coordination polymer particles can be used for encapsulating and delivering drugs. In vitro cancer cell cytotoxicity assays showed that these capsules readily
Selective blockade of protein kinase B protects the rat and human myocardium against ischaemic injury.
Linares-Palomino J, Husainy MA, Lai VK, Dickenson JM, Galiñanes M. J Physiol. 2010 Jun 15;588(Pt 12):2173-91. doi: 10.1113/jphysiol.2010.190462. Epub 2010 Apr 19. https://www.ncbi.nlm.nih.gov/pubmed/?term=7.%09Selective+blockade+of+protein+kinase+B+protects+the+rat+and+human+myocardium+against+ischaemic+injury Abstract Protein kinase B (PKB/Akt) plays a critical role in cell survival but the investigation of its involvement has been limited by the lack of specific pharmacological agents. In this study, using
Aprataxin tumor levels predict response of colorectal cancer patients to irinotecan-based treatment.
Dopeso H, Mateo-Lozano S, Elez E, Landolfi S, Ramos Pascual FJ, Hernández-Losa J, Mazzolini R, Rodrigues P, Bazzocco S, Carreras MJ, Espín E, Armengol M, Wilson AJ, Mariadason JM, Ramon Y Cajal S, Tabernero J, Schwartz S Jr, Arango D. Clinical Cancer Research. 2010 Apr 15;16(8):2375-82 https://www.ncbi.nlm.nih.gov/pubmed/20371676 Abstract PURPOSE: Irinotecan (CPT11) treatment significantly improves the
Oxidative stress after thrombolysis-induced reperfusion in human stroke.
Domínguez C, Delgado P, Vilches A, Martín-Gallán P, Ribó M, Santamarina E, Molina C, Corbeto N, Rodríguez-Sureda V, Rosell A, Alvarez-Sabín J, Montaner J Stroke. 2010 Apr;41(4):653-60. https://www.ncbi.nlm.nih.gov/pubmed/?term=20185784 Abstract BACKGROUND AND PURPOSE: Animal models of transient ischemia suggest that oxygen-derived free radicals produced on reperfusion of ischemic brain could constitute the main cause of reperfusion
Small Gold Nanoparticles Synthesized with Sodium Citrate and Heavy Water: Insights into the Reaction Mechanism
Isaac Ojea-Jiménez, Francisco M Romero, Neus G Bastús, Victor Puntes J. Phys. Chem. C, 2010, 114 (4), pp 1800–1804 http://pubs.acs.org/doi/abs/10.1021/jp9091305 Abstract The effect of solvent isotopic replacement (H for D) on the size of gold nanoparticles (Au NPs) prepared by sodium citrate reduction has been investigated. With increasing replacement of water by deuterium oxide, smaller
Very low-molecular-mass fragments of albumin in the plasma of patients with focal segmental glomerulosclerosis.
Hellin JL, Bech-Serra JJ, Moctezuma EL, Chocron S, Santin S, Madrid A, Vilalta R, Canals F, Torra R, Meseguer A, Nieto JL. Am J Kidney Dis. 2009 Nov;54(5):871-80. https://www.ncbi.nlm.nih.gov/pubmed/?term=Very+low+molecular+weight+fragments+of+albumin+in+the+plasma+of+patients+with+Focal+Segmental+Glomerulosclerosis Abstract BACKGROUND: Primary focal segmental glomerulosclerosis (FSGS) is a glomerular disease that frequently does not respond to treatment and progresses to kidney failure. FSGS can be
The receptor tyrosine kinase EPHB4 has tumor suppressor activities in intestinal tumorigenesis.
Dopeso H, Mateo S, Mazzolini R, Rodrigues P, Lagares-Tena L, Ceron J, Romero J, Esteves M, Landolfi S, Hernández-Losa J, Castaño J, Wilson AJ, Ramon y Cajal S, Mariadason JM, Schwartz Jr S, Arango D. Cancer Research 2009 Sep 15;69(18):7430-8 https://www.ncbi.nlm.nih.gov/pubmed/19738063 Abstract Colorectal cancer is the second cause of cancer-related death in the western world,
Differential proteomic analysis of cyclosporine A-induced toxicity in renal proximal tubule cells.
Puigmulé M, López-Hellin J, Suñé G, Tornavaca O, Camaño S, Tejedor A, Meseguer A. Nephrol Dial Transplant. 2009 Sep;24(9):2672-86. https://www.ncbi.nlm.nih.gov/pubmed/?term=Differential+Proteomic+Analysis+of+Cyclosporine+A-induced+Toxicity+in+Renal+Proximal+Tubule+Cells Abstract BACKGROUND: The use of cyclosporine A (CsA) as a potent immunosuppressant has been limited by its severe nephrotoxic effects. The mechanisms involved are haemodynamic but also related to direct toxic effects of CsA
Evaluation of the ecotoxicity of model nanoparticles
Barrena R, Casals E, Colón J, Font X, Sánchez A, Puntes V. Chemosphere. 2009 May;75(7):850-7. https://www.ncbi.nlm.nih.gov/pubmed/19264345 Abstract Since society at large became aware of the use of nanomaterials in ever growing quantities in consumer products and their presence in the environment, critical interest in the impact of this emerging technology has grown. The main concern
Plasma chitotriosidase activity in multiple sclerosis.
Comabella M, Domínguez C, Rio J, Martín-Gallán P, Vilches A, Vilarrasa N, Espejo C, Montalban X. Clin Immunol. 2009 May;131(2):216-22. doi: 10.1016/j.clim.2008.12.004. Epub 2009 Jan 26. https://www.ncbi.nlm.nih.gov/pubmed/?term=19176289 Abstract A recent study has shown that chitotriosidase (Chit) may play a role in the pathogenesis of multiple sclerosis (MS). Plasma Chit activity was investigated in 219 untreated
Kidney androgen-regulated protein transgenic mice show hypertension and renal alterations mediated by oxidative stress.
Tornavaca O, Pascual G, Barreiro ML, Grande MT, Carretero A, Riera M, Garcia-Arumi E, Bardaji B, González-Núñez M, Montero MA, López-Novoa JM, Meseguer A. Circulation. 2009 Apr 14;119(14):1908-17. doi: 10.1161/CIRCULATIONAHA.108.808543. Epub 2009 Mar 30. https://www.ncbi.nlm.nih.gov/pubmed/?term=Kidney+Androgen-Regulated+Protein+Transgenic+Mice+Show+Hypertension+and+Renal+Alterations+Mediated+by+Oxidative+Stress. Abstract BACKGROUND: Kidney androgen-regulated protein (KAP), a proximal tubule androgen-regulated gene, codes for a protein of unknown function. METHODS AND
Randomized, controlled trial of intramuscular or intracoronary injection of autologous bone marrow cells into scarred myocardium during CABG versus CABG alone
Keng-Leong Ang, Derek Chin, Francisco Leyva, Paul Foley, Chandrashekhar Kubal, Shajil Chalil, Lakshmi Srinivasan, Lizelle Bernhardt, Suzanne Stevens, Lincoln T Shenje and Manuel Galiñanes Nature Clinical Practice Cardiovascular Medicine (2008) 5, 663-670 http://www.nature.com/nrcardio/journal/v5/n10/full/ncpcardio1321.HTML Abstract Background Studies of the transplantation of autologous bone marrow cells (BMCs) in patients with chronic ischemic heart disease have assessed
Distribution and potential toxicity of engineered inorganic nanoparticles and carbon nanostructures in biological systems
Eudald Casals, Socorro Vázquez-Campos, Neus G Bastús, Victor Puntes TrAC Trends in Analytical Chemistry, Volume 27, Issue 8, September 2008, Pages 672–683 http://www.sciencedirect.com/science/article/pii/S0165993608001428 Abstract Biocompatibility, biodistribution, biodegradation, inflammation and interference with cells and normal functioning of organs, among other factors, will determine the toxicity of engineered inorganic nanoparticles and carbon nanostructures, and therefore the extent
Lineage tracing of cardiac explant derived cells.
Shenje LT, Field LJ, Pritchard CA, Guerin CJ, Rubart M, Soonpaa MH, Ang KL, Galiñanes M. PLoS One. 2008 Apr 16;3(4):e1929. doi: 10.1371/journal.pone.0001929. https://www.ncbi.nlm.nih.gov/pubmed/18414652 Abstract AIMS: Cultured cardiac explants produce a heterogeneous population of cells including a distinctive population of refractile cells described here as small round cardiac explant derived cells (EDCs). The aim of
Vacancy coalescence during oxidation of iron nanoparticles
Cabot A, Puntes VF, Shevchenko E, Yin Y, Balcells L, Marcus MA, Hughes SM, Alivisatos AP. J Am Chem Soc. 2007 Aug 29;129(34):10358-60. Epub 2007 Aug 3. https://www.ncbi.nlm.nih.gov/pubmed/?term=Vacancy+coalescence+during+oxidation+of+iron+nanoparticles Abstract In the present work, we analyze the geometry and composition of the nanostructures obtained from the oxidation of iron nanoparticles. The initial oxidation of iron takes
EPHB4 and survival of colorectal cancer patients.
Davalos V, Dopeso H, Castaño J, Wilson AJ, Vilardell F, Romero-Gimenez J, Espín E, Armengol M, Capella G, Mariadason JM, Aaltonen LA, Schwartz Jr. S, Arango D. Cancer Research, 2006; 66(18):8943-8948 https://www.ncbi.nlm.nih.gov/pubmed/16982731 Abstract The family of receptor tyrosine kinases EPH and their Ephrin ligands regulate cell proliferation, migration, and attachment. An important role in colorectal carcinogenesis is
Placental angiogenic growth factors and uterine artery Doppler findings for characterization of different subsets in preeclampsia and in isolated intrauterine growth restriction.
Crispi F, Domínguez C, Llurba E, Martín-Gallán P, Cabero L, Gratacós E. Am J Obstet Gynecol. 2006 Jul;195(1):201-7. Epub 2006 Mar 20. https://www.ncbi.nlm.nih.gov/pubmed/?term=16545329 Abstract OBJECTIVE: The purpose of this study was to evaluate possible relationships between placental markers and endothelial dysfunction in preeclampsia and intrauterine growth restriction. STUDY DESIGN: A prospective study was conducted
The consensus of the task force of the European Society of Cardiology concerning the clinical investigation of the use of autologous adult stem cells for repair of the heart.
Bartunek J, Dimmeler S, Drexler H, Fernández-Avilés F, Galinanes M, Janssens S, Martin J, Mathur A, Menasche P, Priori S, Strauer B, Tendera M, Wijns W, Zeiher A; task force of the European Society of Cardiology. Eur Heart J. 2006 Jun;27(11):1338-40. Epub 2006 Mar 16. https://www.ncbi.nlm.nih.gov/pubmed/16543252 Abstract A task force has been established by
A truncating mutation of HDAC2 in human cancers confers resistance to histone deacetylase inhibition.
Ropero S, Fraga MF, Ballestar E, Hamelin R, Yamamoto H, Boix-Chornet M, Caballero R, Alaminos M, Setien F, Paz MF, Herranz M, Palacios J, Arango D, Orntoft TF, Aaltonen LA, Schwartz S Jr, Esteller M. Nat. Genet., 2006 April;38(5):566-569. http://www.nature.com/ng/journal/v38/n5/full/ng1773.HTML Abstract Disruption of histone acetylation patterns is a common feature of cancer cells, but very
Nanoparticle-mediated local and remote manipulation of protein aggregation.
Kogan MJ1, Bastus NG, Amigo R, Grillo-Bosch D, Araya E, Turiel A, Labarta A, Giralt E, Puntes VF. Nano Lett. 2006 Jan;6(1):110-5. Abstract The local heat delivered by metallic nanoparticles selectively attached to their target can be used as a molecular surgery to safely remove toxic and clogging aggregates. We apply this principle to protein
Nanocrystalline CeO2 Increases the Activity of Au for CO Oxidation by Two Orders of Magnitude
Silvio Carrettin, Patricia Concepción, Avelino Corma, José M López Nieto, Victor F Puntes Angewandte Chemie International Edition 43(19):2538-40 · May 2004 http://onlinelibrary.wiley.com/doi/10.1002/anie.200353570/full Abstract The smaller the better: Depositing gold on nanocrystalline particles of cerium oxide, instead of a regular cerium oxide support, increases the activity of the resulting catalyst by two orders of magnitude for the
Collective behaviour in two-dimensional cobalt nanoparticle assemblies observed by magnetic force microscopy.
Puntes VF, Gorostiza P, Aruguete DM, Bastus NG, Alivisatos AP. Nat Mater. 2004 Apr;3(4):263-8. Epub 2004 Mar 28. https://www.ncbi.nlm.nih.gov/pubmed/?term=Collective+behaviour+in+two-dimensional+cobalt+nanoparticle+assemblies+observed+by+magnetic+force+microscopy Abstract The use of magnetic nanoparticles in the development of ultra-high-density recording media is the subject of intense research. Much of the attention of this research is devoted to the stability of magnetic moments, often neglecting
Synthesis of hcp-Co nanodisks
Victor F. Puntes , Daniela Zanchet , Can K. Erdonmez , and A. Paul Alivisatos Journal of the American Chemical Society, 2002, 124 (43), pp 12874–12880 http://pubs.acs.org/doi/abs/10.1021/ja027262g Abstract hcp Co disk-shaped nanocrystals were obtained by rapid decomposition of cobalt carbonyl in the presence of linear amines. Other surfactants, in addition to the amines, like phosphine oxides and oleic acid were used to improve size dispersion,
Synthesis, self-assembly, and magnetic behavior of a two-dimensional superlattice of single-crystal ε-Co nanoparticles
Victor F. Puntes, Kannan M. Krishnan and Paul Alivisatos Applied Physics Letters. 78, 2187 (2001) http://aip.scitation.org/doi/abs/10.1063/1.1362333 ABSTRACT A method of producing high-quality magnetic colloidal dispersions by the rapidpyrolysis of cobalt carbonyl in an inert atmosphere was employed to produce monodispersed, stabilized, defect-free ε-cobalt nanocrystals, with spherical shapes and sizes ranging from 3 to 17 nm. The size distribution and the shape of the nanocrystals were
Colloidal nanocrystal shape and size control: the case of cobalt
Victor F. Puntes, Kannan M. Krishnan, A. Paul Alivisatos Science 16 Mar 2001: Vol. 291, Issue 5511, pp. 2115-2117 http://science.sciencemag.org/content/291/5511/2115 Abstract We show that a relatively simple approach for controlling the colloidal synthesis of anisotropic cadmium selenide semiconductor nanorods can be extended to the size-controlled preparation of magnetic cobalt nanorods as well as spherically shaped