Electrochemical biosensing of non-electroactive targets using ferrocene-labeled magnetic particles and CNT wiring

Z Herrasti, R Olivé-Monllau, FX Muñoz-Pascual, F Martínez, E Baldrich

Analyst. 2014 Mar 21;139(6):1334-9.

https://www.ncbi.nlm.nih.gov/pubmed/?term=Electrochemical+biosensing+of+non-electroactive+targets+using+ferrocene-labeled+magnetic+particles+and+CNT+wiring

Abstract

We show that target binding onto ferrocene-modified magnetic microparticles (MP-Fc) promotes physical sheltering of the labels. This can be measured electrochemically by CNT wiring, which enhances ten-fold the signals registered compared to direct detection of the MPs alone. As a proof of concept, detection of detergents and antibodies is accomplished. In these preliminary experiments, random binding of 0.01% Tween 20 onto MP-Fc was detectable both voltametrically and impedimetrically after a 2 min incubation. Furthermore, affinity capture of 4 μg mL(-1) of biotinylated antibody by streptavidin MP-Fc could be measured in less than 30 min and even in the presence of 1 mg mL(-1) of BSA.